Performance of Trichoderma koningii and Bacillus sp. as Potential Biocontrol Agents against Fusarium Wilt (Fusarium oxysporum) of Sweetpotato (Ipomoea batatas)
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Abstract
Fusarium wilt (Fusarium oxysporum) is now becoming a big concern in Cordillera Administrative Region, Philippines affecting different crops including sweetpotato. In effort to help find solution to this problem, the study documented the biological control ability of Trichoderma koningii and Bacillus sp. against Fusarium wilt of sweetpotato through bioassay and pot experiment. Result of the bioassay test in the laboratory showed significant results. The growth of F. oxysporum was inhibited by Benomyl (reference fungicide) and T. koningii. The widest inhibition zone of 29 mm after three days was observed in Benomyl followed by T. koningii with 20 mm. Bacillus sp. gave the least inhibition zone of 3 mm. In the pot experiment, sweetpotato grown in soils amended with T. koningii had the lowest percent infection and lowest severity of diseases. At the end of the cropping season, populations of Bacillus and F. oxysporum decreased while T. koningii increased. The highest reduction of Fusarium spores from 1.8 x 106 to 9.50 x 105 was noted in soils amended with T. koningii, followed by Benomyl from 1.8 x 106 to 1.40 x 105 cfu/ml. Results consistently showed better performance of T. koningii as sole inoculant in inhibiting Fusarium wilt and even better than Benomyl, thus should be encouraged for farmers to adopt.
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